Mannitol solution (0.5 M)

YJ Yeong Yeop Jeong
HL Hun-Young Lee
SK Suk Weon Kim
YN Yoo-Sun Noh
PS Pil Joon Seo
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To prepare 0.5 M mannitol, 91 g d-mannitol and 0.42 g MES·H2O were dissolved in 1 L ddH2O. The pH was adjusted to 5.8 with 2 M NaOH and/or 1 M HCl. The solution was sterilized in an autoclave at 121 °C for 10 min and stored at room temperature.

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