Fluorescence in situ hybridization (FISH)

QL Qi Lv
GW Gangmin Wang
YZ Yinan Zhang
AS Aijun Shen
JT Junjun Tang
YS Yi Sun
CM Chunhui Ma
PW Peijun Wang
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FISH was performed to investigate the distribution of circAGAP1 and miR-15a-5p in ACHN and A498 cells. In brief, Cy3-labeled probes targeting miR-15a-5p (hsa-miR-15a-5p-CY3: CAAACCATTATGTGCTGCTA) and FITC-labeled probes targeting circAGAP1 (hsa_circ_0058792-FITC: TTCACGAAGGCATCGTCCTGGAA) were designed. FISH analysis was performed using a Fluorescent In Situ Hybridization Kit (GENESEED, Guangzhou, China) according to the manufacturer’s instructions. Nuclei were counterstained with DAPI (Beyotime, China). Images were obtained under laser scanning confocal microscopy (Leica, Germany).

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