For soil preparation, A. argyi powder was mixed into sand soil at the ratio of 100:0, 100:2, 100:4, and 100:8, separately. 800 g soil poured into plastic bowls with upper mouth diameter of 14.5 cm, lower mouth diameter of 10.5 cm and height of 9 cm. Then equal amount of water was added into each treatment group to soak the soil and seeds were sown 2 days later. Fifty seeds of B. pekinensis, L. sativa, O. sativa, P. oleracea, O. corniculata and S. viridis were sown independently in each bowl with three biological repeats. The germination rates and plant heights of B. pekinensis, L. sativa and O. sativa were measured at 9 days post treatment, and same data were recorded in P. oleracea, O. corniculata, and S. viridis at 13 days post treatment.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.