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Flow cytometry

TD Tatyana Dubich
AD Anne Dittrich
KB Kristine Bousset
RG Robert Geffers
GB Guntram Büsche
MK Mario Köster
HH Hansjörg Hauser
TS Thomas F. Schulz
DW Dagmar Wirth
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2D cultured cells and 3D spheroids were dissociated with trypsin/EDTA; engrafted cells were isolated as described below. Cells were resuspended in PBS supplemented with 2% FCS. GFP and BFP expression was assessed by flow cytometric analysis (BD™ LSRII analyzer). Non-infected cells served as a negative control. The data were processed with the FlowJo v10 software.

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