Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)

AC Ai-Ping Chang
JQ Jin Qian
HL Hui Li
YW Ying-Lu Wang
JL Jie-Ying Lin
QH Qiao-Mei He
YS Ya-Ling Shen
HZ Hu Zhu
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Total protein and purified enzymes (including mutant proteins) were analyzed by SDS-PAGE using an 8% acrylamide slab gel. The protein was stained with Coomassie Brilliant Blue G-250. The molecular weight was determined using standard molecular weight markers as a reference.

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