2.4.1. Rotarod test

Mice were placed on a rotating rod that accelerates linearly from 4 to 40 rpm (Rotarod, Ugo Basile), and the average time spent on the rotating rod for three consecutive trials was calculated for each mouse. Mice were allowed to run for a maximum of 5 min with a 5 min rest period between each run, and the latency to fall was recorded for three consecutive trials. The average of the three trials was taken as the data point for each mouse at the age tested, and the performance of different groups are reported as the mean ± SEM.