Data are expressed as means ± SEMs. Data shown are representative of one of two or three independent experiments. The comparison was performed by GraphPad Prism V5 (GraphPad Software). Mann-Whitney U test was used to analyze the comparison of IL-6 concentration in MPN patients with/without the JAK2V617F mutation. The student’s t-test was used to analyze the comparison of IL-6 concentrations in the knockdown or overexpression system. One-way analysis of variance (ANOVA) was used to analyze the comparison of empty-, JAK2-, or JAK2V617F-expressing macrophages. Upon stimuli, two-way ANOVA was used for comparison of empty-, JAK2-, or JAK2V617F-expressing macrophages.
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