siRNA Knockdown of GAPLINC.

On day 3 of differentiation, human MDMs were transfected with 25 nM siRNA using Lipofectamine 2,000 (Life Technologies, 11668027). Silencer Select Negative-Control siRNA (Life Technologies) was used as a control. Transfection was allowed to proceed for 72 h, and then RNA was isolated as previously described to create sequencing libraries and assess kd efficiency by qPCR. The siRNA sequences are as follows: siGAPLINC_1, GAA​GAA​AAC​CUG​GAA​GUU​ATT; siGAPLINC_2, GAA​CAA​UGA​AGA​AAC​CAA​ATT; and siGAPLINC_3, GGA​UUA​UGU​GGU​UUG​UAA​ATT; >GAPLINC (ENST00000579007.5): AGC​CAA​TGC​CTG​AAA​TAA​TGA​ACT​CCT​CCA​AGG​CAA​GAA​ATC​TGT​TTT​GAA​GCT​TCT​CTG​CGT​TCA​CAC​ACA​GCA​GCC​TGG​TTT​CCT​GGA​AGG​GCA​TTT​TCC​ACA​TTG​TGC​GTT​ATG​GAT​GAT​CAT​CCC​AGG​CAT​CAG​GTG​TGA​AGC​CCT​GCA​TCC​ACA​TCC​AGG​GGC​TAT​CAA​ATC​TCT​CTG​CAA​AAG​GAG​AAG​CTG​GAC​TCA​GGC​ACG​TTT​ACA​GTG​ATG​TGT​ATG​CAG​TTC​TGT​GAA​GGA​AGC​TGC​AGA​AGA​AAA​AGG​TGA​AAG​AGG​TG.

AGG​AAG​CTG​CAGAA​GAA​AAC​CTG​GAA​GTT​AGCA​GAG​CTT​GAT​CCA​GAG​GTT​TAA​GGA​AAG​AAG​CCA​TCT​CCA​TAA​CAT​AAA​AGT​GCA​AGG​TGA​AGC​AGC​AAG​TGC​TGA​TGG​GGA​AGC​TGC​AGC​AAG​TCA​TCC​AGA​AGA​TCT​TGC​TAA​GGG​TAT​GCA​CAG​ATG​TGG​AAA​CAG​GAA​CTG​ATG​TGT​CCA​TTA​CAC​CAC​TAG​GAC.

AGA​GGC​CAGAA​CAA​TGA​AGA​AAC​CAA​ATAC​TTG​GAA​GAG​GGT​AGA​GAT​AAT​GAA​TGG​AGT​CCA​AGA.

GCC​CTG​ATT​GTG​CCA​TAA​ATG​TCC​AGA​TAA​TTC​CAT​ACC​TGAGGA​TTA​TGT​GGT​TTG​TAAACT​TGG​CAC​TTA​GAA​GAA​CCA​ATA​AAA​TCA​TGT​TAT​AGT​TTC​AA.