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SMFA

SS Stephen W. Scally
BM Brandon McLeod
AB Alexandre Bosch
KM Kazutoyo Miura
QL Qi Liang
SC Sean Carroll
SR Sini Reponen
NN Ngan Nguyen
EG Eldar Giladi
SR Sebastian Rämisch
VY Vidadi Yusibov
AB Allan Bradley
FL Franck Lemiale
WS William R. Schief
DE Daniel Emerling
PK Paul Kellam
CK C. Richter King
JJ Jean-Philippe Julien
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Antibodies were buffer exchanged in phosphate-buffered saline (PBS) and tested at multiple concentrations in SMFA (Anopheles stephensi (Nijmegen strain)) as previously described26. Four independent feeding experiments were conducted. In the first two feeds (Feed #1 and #2), 1245 and 1269 mAbs were tested alone (n = 20 mosquitoes per condition). In Feed #3 and #4, the two mAbs were examined either alone (n = 20) or as mixtures. A mixture condition of 1245 (31 μg/ml) and 1269 (4 μg/ml) was tested once in Feed #3 (n = 20), and the other three conditions (45-7, 73-13, and 132-27 μg/ml for 1245–1269, respectively) were tested in two feeding experiments (n = 20 in Feed #3, and n = 40 in Feed #4).

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