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Pretreatment for the fecal samples and RNA extraction

RC Ranran Cao
LB Lirong Bao
MP Ming Pan
CZ Cheng Zhang
HL Hongyu Liao
LL Li Liu
YL Yan Li
ML Mingyuan Li
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To study the influence of different pretreatment methods on SARS-CoV-2 NAT results, 38 fecal samples were selected randomly to suspend in two ways. To be specific, 200 mg of stool was suspended in a 15 mL tube containing 2 mL PBS, 2 mL Trizol (Trizol@ Reagent, invitrogen, USA), respectively. The mixture was stirred gently and mixed. Keep still for 10 min and then supernatant were transferred to a new tube. Depending on the NAT results of the 38 randomly selected fecal samples, the remaining 52 samples were suspended in Trizol. 200 μL supernatant were used for RNA isolation by the NP968 Nucleic Acid Extraction System (Xi’an Tianlong Science & Technology Co., LTD, Xi’an, China). Extracted RNA was stored at − 20 °C until use.

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