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Estimation of the ash content of chitin and chitosan
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The ash content of each chitin and chitosan sample was gravimetrically estimated after the pyrolysis of 1 g in a muffle furnace at 650 °C for 5 h. This procedure was done in triplicates and the mean ash content computed. The ash content was computed as a fraction of mass of the residue (MR) and mass of the sample (MS) using the formula (1) that follows;
where MS and MR are the weights (in grams) of the initial sample of the sample and residue respectively74.
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