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In vivo xenograft model

TY Tingting Yan
CS Chaoqin Shen
PJ Penglei Jiang
CY Chenyang Yu
FG Fangfang Guo
XT Xianglong Tian
XZ Xiaoqiang Zhu
SL Shiyuan Lu
BH Bingshe Han
MZ Ming Zhong
JC Jinxian Chen
QL Qiang Liu
YC Yingxuan Chen
JZ Junfang Zhang
JH Jie Hong
HC Haoyan Chen
JF Jing-Yuan Fang
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Four-week-old male BALB/c nude mice were purchased from Experimental Animal Center of SIBS. DLD-1 cells were injected subcutaneously in the groin or armpit into the mice to establish the CRC xenograft model (for the tumor formation assay, cells were inoculated in the groin of mice; for the 18F-FDG PET detection assay, which were repeated for two times, cells were inoculated in the groin or the armpit of mice, separately). Five days after inoculation, the mice were injected with indicated adenovirus by multipoint intratumoral injection every 2 days. The mice were sacrificed 3 weeks after the inoculation of tumor. Tumor volume (mm3) was assessed by the formula: tumor volume (mm3) = longer diameter × shorter diameter2/2.

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