2.10. Statistical Analysis

Statistical analysis was performed using the GraphPad Prism 8 software. Python 3.6 and module Seaborn Pandas were also used to analyze and plot the data. The data between the two groups were analyzed by two-tailed paired or unpaired Student t-test and expressed as mean ± standard deviation, and the chi-squared test was applied to examine some of the clinical characteristic parameters. The Pearson test was performed to determine the correlation between different expression profiles on CD4⁺ and CD8⁺ T cells, p < 0.05 for significant differences (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001).