2.5. Neurobehavioral Deficits

Neurological function was tested at 3 days after operation by investigators who were blinded to the divided groups. All procedures were performed as we previously reported [10]. Briefly, for the beam walking test, mice were placed midway on a 90 cm long, 1.5 cm wide, and 40 cm high rod with two platforms at both ends. To avoid falling, mice moved along the horizontal rod to reach anyone of the platforms. The scores were recorded by measuring the distance and time of mice to fall as we previously described [10]. For the corner turn test, mice were left in a 30° corner. To exit, mice will turn either left or right. The percentages of turning left were recorded. For the forelimb placing test, the mouse vibrissae were stimulated to observe the respective response of left paw placing. The percentages of left paw placing were recorded. For the Rotarod test, mice were trained three times a day for a total of 3 d presurgery. Each trial persisted for 5 min with a 2 h rest interval. According to the animals' habitation, the trail speed accelerated gradually from 4 to 40 rpm. After ICH induction, mice were still trained as before and the latency to fall was recorded on day 3.