Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Construction of Fab expression plasmids
Request a Protocol
Ask a
question
Favorite
Heavy chain expression plasmids of specific antibodies were used as templates to amplify the first fragment, heavy chain vector include the variable region and CH1 until Kabat amino acid number 233. The second fragment of thrombin cleavage site and twin-Strep-tag with overlapping ends to the first fragment were amplified. The two fragments were ligated by Gibson assembly to make the Fab heavy chain expression plasmid.
Copyright and License information: ©2021 The Authors
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
