SgRNA design and synthesis

GL Guichun Liu
WL Wei Liu
RZ Ruoping Zhao
JH Jinwu He
ZD Zhiwei Dong
LC Lei Chen
WW Wenting Wan
ZC Zhou Chang
WW Wen Wang
XL Xueyan Li
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The sgRNA target sites were designed based on principle 5′-N20NGG-3′ (Additional file 1: Table S4) [86]. The dsDNA template for sgRNA production was generated according to our previously protocol [2, 75]. Recombinant Cas9 protein (PNA Bio Inc., CA, USA) were purchased directly.

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