To construct the protein-protein interaction networks (PPINs) of CsARGs, the ortholog groups of CsARGs must be identified firstly from Arabidopsis due to there is no PPIs information of tea plant in the current PPI database. Here, the Blast search column in Inparanoid (http://inparanoid.sbc.su.se/cgi-bin/index.cgi) is used to determine the ortholog groups of CsARGs, and we only picked the orthologs originated from Arabidopsis to construct PPINs. Then, STRING (https://string-db.org, Ver10.5) was used to construct the PPINs of the ortholog groups. In brief, all geneID of obtained orthologs were uploaded into the ‘Multiple Proteins by Names/Identifiers’ column, and Arabidopsis thaliana was chosen as a reference for blast searching. Then, the matched STRING proteins were chosen to build PPINs. Finally, the result was uploaded into Cystoscape v3.7.2 to draw the interaction map.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
 Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.