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Human breast cancer xenograft model

YT Yuelin Tong
ZL Zhongwei Li
YW Yikuan Wu
SZ Shenglong Zhu
KL Keke Lu
ZH Zhao He
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This study was approved by the ethics committee of Jiangnan University with protocol number SYXK2016-0045. Female BALB/c nude mice, 4 weeks of age, were purchased from Lingchang Biotechnology Co., Ltd. (Shanghai, China). All the experiments were in accordance with national institution guidelines. MDA-MB-231 cells (1 × 106) suspending in medium were subcutaneously injected in the right flank of mice. After 1 week of injection, the mice bearing tumor were randomly subdivided into 2 groups and administered via diet either with vehicle (model group) or 0.5% (w/w diet) LAE. Tumor dimensions were measured with vernier caliper and tumor volumes were estimated by the formula: length × width^2 × 0.5. Body weights and tumor growth were recorded twice a week. The mice were sacrificed after 56 days according to the appearance of metastasis, and visible metastatic tumors were counted and subjected to further analysis.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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