The transfected LAD cells were seeded into 96-well plates at a density of 3 × 103 cells/well, then, after 0, 24, 48 and 72 h, CCK-8 solution (Signalway Antibody, College Park, MD, USA) was added to each well and incubated for 1 h. The absorbance (optical density value) was measured at a wavelength of 450 nm on a microplate spectrophotometer (Thermo Fisher Scientific).
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