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MTT assay, transwell assay, and cloning formation assay

SC Siming Chen
MY Mengxue Yu
LJ Lingao Ju
GW Gang Wang
KQ Kaiyu Qian
YX Yu Xiao
XW Xinghuan Wang
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For MTT assay, after 36 h of transfection, cells were planted into 96-well plates in different groups, then absorbance measurements were used to assess cell viability at different times. For the cloning formation assay, 1000 transfected cells were grown in a six-well culture dish, fixed and stained 14 days later. For transwell assay, the transfected cells were seeded into the upper chambers (Corning), and 600 µL medium containing 10 % FBS was added into the lower chambers. After incubation for 24 h, the cells were fixed and stained.

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