NO production assay

JH Jia-Hui He
RL Rong-Pei Liu
YP Yi-Man Peng
QG Qing Guo
LZ Lan-Bing Zhu
YL Yi-Zhi Lian
BH Bei-Lei Hu
HF Hui-Hui Fan
XZ Xiong Zhang
JZ Jian-Hong Zhu
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Medium nitrite was measured as an indicator of NO production [24]. In brief, 50 μl of supernatant was mixed with an equal volume of Griess reagent I, followed by an addition of 50 μl of Griess reagent II (Beyotime, Shanghai, China) at room temperature. Absorbance was immediately measured at 540 nm. The concentration of each sample was calculated from a standard curve generated using sodium nitrite.

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