Histological methods to assess intervertebral fusion

WL Weigang Li
CH Chunwei Huang
TM Tian Ma
JW Jiachen Wang
WL Wenbin Liu
JY Jiyuan Yan
GS Gaohong Sheng
RZ Ruizhuo Zhang
HW Hua Wu
CL Chaoxu Liu
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Following all the CT scans, the specimens were immersed in 10% EDTA decalcification solution. Then, the decalcified specimens were treated with gradient alcohol dehydration and embedded in paraffin to make tissue sections. Hematoxylin and eosin (HE) staining and Masson staining were performed to analyze the new bone formation in the interbody space. New bone area fraction calculated as new bone area/defect area within the defect of each section was obtained using ImageJ software (n = 6).

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