Following all the CT scans, the specimens were immersed in 10% EDTA decalcification solution. Then, the decalcified specimens were treated with gradient alcohol dehydration and embedded in paraffin to make tissue sections. Hematoxylin and eosin (HE) staining and Masson staining were performed to analyze the new bone formation in the interbody space. New bone area fraction calculated as new bone area/defect area within the defect of each section was obtained using ImageJ software (n = 6).
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