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Cell viability measurement

JH Jia-Hui He
RL Rong-Pei Liu
YP Yi-Man Peng
QG Qing Guo
LZ Lan-Bing Zhu
YL Yi-Zhi Lian
BH Bei-Lei Hu
HF Hui-Hui Fan
XZ Xiong Zhang
JZ Jian-Hong Zhu
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Cell viability was determined by the tetrazolium salt 3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide (MTT; Sigma, St. Louis, MO, USA) assay [23]. Primary astrocytes were seeded in triplicates in 24-well plates at 1 × 105 per well and cultured for 7 days in the NB+ medium. Astrocytes were treated with different antidepressants at different concentrations for 24 h following overnight starvation. Thereafter, MTT was added into each well and incubated at 37 °C for 1 h. The resulting formazan crystals were dissolved in dimethyl sulfoxide (Sigma, St. Louis, MO, USA). Optical density was measured at 490 nm.

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