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Preparation of PCL/nHA scaffolds

WL Weigang Li
CH Chunwei Huang
TM Tian Ma
JW Jiachen Wang
WL Wenbin Liu
JY Jiyuan Yan
GS Gaohong Sheng
RZ Ruizhuo Zhang
HW Hua Wu
CL Chaoxu Liu
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PCL and nHA were mixed in dichloromethane at a ratio of 7:3. After the dichloromethane was partially volatilized, the mixture was printed into a porous scaffold with a fused deposition modeling (FDM) 3D printer. The manufactured scaffolds were divided into two types: one is a square with the side length of 8 mm, another is a disc with a diameter of 4 mm, and the thickness of all scaffolds is 1 mm. The square scaffold serves as a carrier for cells in vitro while the disc scaffold is used in vivo. Then, the scaffolds were placed in a fume hood overnight to completely volatilize the residual dichloromethane. Finally, these scaffolds were sterilized by ethylene oxide in the hospital.

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