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Fluorescence measurement

PV Phuong N. L. VO
HL Hyang-Mi LEE
JR Jun REN
DN Dokyun NA
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To confirm the expression level of hfq variants, the gfp gene was cloned downstream of the hfq coding sequence as a fusion protein. The fluorescence intensity of GFP was measured by Guava Easycyte HT BG Flow Cytometer (Millipore, Billerica, MA, USA) to determine the expression level of hfq variants. For fluorescence measurement, harvest cells were washed and resuspended in 1× phosphate-buffered saline (PBS) buffer at pH 7.4 (Sigma-Aldrich, St. Louis, USA), and subsequently diluted 1000 times with 1× PBS buffer. All experiments were performed in triplicate.

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