At selected PDs throughout their proliferative lifespans, DPSCs expanded with or without H2O2 (0–200 μM) were maintained in 6-well plates as above, until 80–90% confluent. Following DNA purification [12], telomere length analyses were performed using the TeloTAGGG Telomere Restriction Fragment (TRF) Length Assay Kit (Roche, Welwyn Garden City, UK), per the manufacturer’s instructions. A digoxigenin (DIG)-labeled molecular weight marker (kb, in Kit) and positive DIG-labeled control DNA sample (CTRL, in Kit) were also included. Mean telomere lengths were calculated from Southern blot images via ImageJ® Software [12].
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