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PBMC isolation and stimulation
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Cells were isolated by density centrifugation on Ficoll‐Paque (GE Healthcare, Chalfont St Giles, UK), washed three times in PBS and resuspended in culture medium. After isolation, 5 × 105 PBMCs were added to a round bottom 96‐well plate (Greiner, Kremsmünster, Austria). Pam3Cys (10 μg mL−1) or LPS (10 ng mL−1) was added for 24 h at 37°C and 5% CO2.
Copyright and License information: ©2021 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology Inc.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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