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ESI-MS/MS analysis of cells

LI Lishann M. Ingram
MF Morgan C. Finnerty
MM Maryam Mansoura
CC Chau-Wen Chou
BC Brian S. Cummings
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Samples were run in triplicate (n = 3) and the most abundant species were defined as the core lipid pool [17]. Lipid extracts (500 pmol/μL) were prepared by reconstitution in chloroform: methanol (2:1, v/v). ESI-MS was performed as previously described [18–20] using an LCQ Deca ion-mass spectrometer (LCQ Finnigan mass spectrometer (Thermo Fisher-Fenning Institute, CA, USA)) with a nitrogen drying gas flow-rate of 81/min at 350 °C and a nebulizer pressure of 30 psi. The scanning range was from 200 to 1000 m/z on 5 μL of the samples scanned in the positive and negative mode for 2.5 min with a mobile phase of acetonitrile; methanol; water (2:3:1) in 0.1% ammonium formate.

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