Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Tissue preparation for fISH
Request a Protocol
Ask a
question
Favorite
WT, Tg21 mice of both sexes at postnatal ages P7, P11, P21, and P60, and GAD65-cre-positive, EPORfx/fx, and GAD65-cre-negative, EPORfx/fx mice at postnatal age P11, were deeply anesthetized with an intraperitoneal injection of sodium pentobarbital (50 mg/kg) followed by decapitation and dissection of brain tissue on ice. Hemispheres where then frozen on dry ice and stored at −80°C until use. Serial brain sections of 10 μm were cut using a cryostat (Leica Biosystems), mounted on Superfrost slides and stored at −80°C.
Copyright and License information: ©2021 Khalid et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
