Drugs and viral vectors

(-)Nicotine tartrate (MP Biomedicals) was dissolved in 0.9% sterile saline and adjusted to pH 7.2–7.4 with 1 m NaOH. The final stock concentration was 0.2 mg/ml free base, which was adjusted for body weight to achieve an infusion concentration of 0.02 mg/kg/ml. CNO was purchased from Sigma-Aldrich and diluted in ACSF purchased from Tocris Biotech to 0.1 mg/ml. Heparin, xylazine (100 mg/ml), cefazolin and meloxicam used at 10 U/ml, 8 mg/kg/ml, 100 mg/kg/ml, and 1 mg/kg in 0.9% sterile saline, respectively.