Cellular uptake of 125I-AV3

The cellular uptake difference of ¹²⁵I-AV3 between PSCs and SW1990-CM-treated PSCs was compared. PSCs or SW1990-CM-treated PSCs were seeded in a 6-well plate at a density of 2 × 10⁵ cells/well, and about 50 μCi ¹²⁵I-AV3 was added into each well. Cells were further cultured at 4 °C for 0.5 h, 2 h or 4 h, and at each time point the free ¹²⁵I-AV3 was removed by washing cells twice with PBS. Then, 0.1 M NaOH was added to solubilize the cells, and the cell-bound radioactivity (counts per minute, CPM) of well was measured using a γ-counter.