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Enzyme-Linked Immunosorbent Assay (ELISA)

SW Shuang Wu
YW Yining Wang
HD Haixiang Ding
NS Ningxia Sun
YL Yan Liu
LW Liang Wang
FS Fei Sheng
HZ Honghong Zhang
FY Fu Yang
WL Wen Li
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Hormone levels in mice were monitored dynamically before and after ovariectomy and ovary transplantation by ELISA. Venous blood samples were collected from the eye socket veins in mice. Serum was extracted at three time points: before castration, after castration, and 30 days after transplantation. Serum was separated and stored at −80°C until use. Repeated freeze/thaw cycles were avoided. Serum FSH and AMH levels were measured by using an ELISA kit for mice following the manufacturer’s instructions (Westang, Shanghai, China).

Copyright and License information:  ©2021 Wu, Wang, Ding, Sun, Liu, Wang, Sheng, Zhang, Yang and Li.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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