Sample leaves (0.1 g, 3-week-old Arabidopsis seedlings) of the transgenic and control lines (under natural growth conditions and subjected to stress treatments) were put into 10 mL of distilled water. To measure the initial electric conductance (S1) by FG3-B FiveGoTM conductivity meter (METTLER TOLEDO, Switzerland) (25 °C), a vacuum (test tube) was applied for 30 min, after which the vacuum pressure surged for 2 h. The applied temperature was then increased (100 °C) for 30 min, after which it was reduced to room temperature (25 °C), at which point the final electric conductance (S2) was determined. The relative electrical conductivity was evaluated as S1 × 100/S2.
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