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Bone marrow cells were collected in RPMI-1640 media from tibias and femurs of BALB/c mice. Red blood cells were lysed in Tris-ammonium chloride for 2 min, and bone marrow cells were seeded in 6-well plates precoated with recombinant mouse FLT3 ligand (R&D Systems, Minneapolis, MN, USA). The suspension cells were collected for co-culture with tumor cells.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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