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Bacterial motility was determined using a soft agar plate according to a method described previously (Luo et al., 2016). The OD600 of bacterial cultures grown overnight in LB was adjusted to 0.3, and plates of LB agar (0.3% agar) were seeded using 1 μl of the culture suspension. After incubating the plates at 28°C for 24 h, the diameters of the bacterial colonies were measured. Each experiment was performed in triplicate.
Copyright and License information: ©2021 Xu, Li, Qi, Chen, Qin, Zheng and Jiang
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