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Transwell Migration Assay

SH Suyu Hao
JZ Jun Zhu
XZ Xinyue Zhang
JQ Jingyue Qiu
QX Qin Xuan
LY Liping Ye
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2 × 105 cells were suspended in serum-free medium and then placed in the superior compartment of 8 μm-well Transwells (BD Biosciences, San José, CA, United States). The lower compartment with 10% FBS was regarded as a chemical attractant. After cells incubating at 37°C for 2 days and staining for 15 min, 5 fields of view (× 200) were stochastically chosen under the microscope to calculate translocated cells.

Copyright and License information:  ©2021 Hao, Zhu, Zhang, Qiu, Xuan and Ye.
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