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Mouthwash sample collection, processing, storage and DNA extraction

IG Ian Ganly
ZP Zhiheng Pei
YH Yuhan Hao
YM Yingfei Ma
MR Matthew Rosenthal
ZW Zhenglin Wu
JM Jocelyn Migliacci
BH Bin Huang
NK Nora Katabi
WT Wenzhi Tseng
SB Stuart Brown
YT Yi-Wei Tang
LY Liying Yang
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The participants rinsed their mouth vigorously with 10 ml sterile saline for 30 s and then mouthwash collected in a 50 cc falcon canical flask container. After centrifugation at 3120×g for 20 min, supernatants were decanted and then the cell pellets were transferred into a 2-ml Eppendorf tube and stored at − 80 °C freezer for further study. All oral rinse specimens were taken prior to surgical resection of the OC-SCC. These samples were de-identified and coded. Using the MoBio method, we successfully extracted DNA from all 100 oral wash samples. DNA yield was measured by the Nanodrop method.

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