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RT-qPCR

HZ Hailing Zhang
KZ Ke Zhang
ZX Zhen Xu
ZC Zhilong Chen
QW Qian Wang
CW Chenyang Wang
JC Jinquan Cui
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The TRIzol Reagent (Invitrogen) was used for extract total RNA from tissues and cells. RNA was reversely transcribed into cDNA using a miScript II RT kit (TaKaRa, Biotechnology Ltd., Dalian, China) and then the real-time qPCR was performed using a Premix Ex TaqTM II (TaKaRa) on an ABI PRISM 7500 real-time PCR System. Relative gene expression was determined by the 2-ΔΔCt method. The primers are listed in Table 1, in which U6 and GAPDH were set as the internal references for miRNA and mRNAs, respectively.

Primer sequences for RT-qPCR

Note: RT-qPCR reverse transcription quantitative polymerase chain reaction, Bcl-2 B-cell lymphoma-2, Bax Bcl-2-associated X, KDM4B Lysine (K)-specific demethylase 4B, PLK1 Polo-like kinase 1, GAPDH glyceraldehyde-3-phosphate dehydrogenase, F forward, R reverse

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