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Plasma corticosterone measurement

HS Hirotaka Shoji
TM Tsuyoshi Miyakawa
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In the first, second, and third cohorts of mice, immediately after the elevated plus maze test (within approximately 1 min), blood was collected from the facial vein or submandibular vein using a Goldenrod Animal Lancet (MEDIpoint, Inc., NY, USA) into tubes containing 1 unit of sodium heparin (Wako Pure Chemical Industries Ltd., Osaka, Japan) and kept on ice. In the fourth cohort of mice, at least one hour after acclimation to the testing room where an elevated plus maze apparatus was set, blood was collected to measure basal levels of plasma corticosterone. The blood samples were centrifuged at 3000×g for 10 min at 4 °C. Supernatants were collected and stored at − 80 °C until measurement. Plasma corticosterone (CORT) concentrations were determined using a correlate-enzyme immunoassay kit (Assay Designs Inc., MI, USA) according to the manufacturer's protocol.

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