SRB assay was used to measure growth inhibition in cells. MDA-MB-231 cells (and SUM159 cells) (CRISPR scrambled and stable KO cells) were grown in DMEM complete medium (and F12 HAM’s complete medium, respectively) (2500 cells/well) in a 96-well plate and allowed to attach for 24 h. The cells were then treated with a dose range of celecoxib for 96 h. After treatment, the cells were fixed with 50% trichloroacetic acid (TCA) for 2 h at 4 °C, rinsed with water 4 times, stained with 0.4% SRB for 1 h and rinsed with 1% acetic acid. After air dry overnight, the SRB dye was solubilized with 10 mM Tris base and the plates were read at 490 nm using a microplate reader. The results were analyzed and graphed using GraphPad Prism 6.0 (GraphPadSoftware, Inc., San Diego, CA).
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