Data availability.

EB Emily Bordeleau
PS Peter J. Stogios
EE Elena Evdokimova
KK Kalinka Koteva
AS Alexei Savchenko
GW Gerard D. Wright
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PDB validation reports for crystal structures obtained in this study have been submitted with the manuscript. Accession numbers are as follows: 7JM0 (ApmA apoenzyme), 7JM1 (ApmA complex with acetyl-CoA), and 7J6M2 (ApmA complex with apramycin).

13C NMR for 2′-acetyl-apramycin. Download FIG S5, PDF file, 0.1 MB.

1H-1H correlation spectroscopy (COSY) NMR for 2′-acetyl-apramycin. Download FIG S6, PDF file, 0.1 MB.

1H-13C heteronuclear single quantum coherence (HSQC) for 2′-acetyl-apramycin. Download FIG S7, PDF file, 0.1 MB.

1H-13C HMBC for 2′-acetyl-apramycin. Download FIG S8, PDF file, 0.1 MB.

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