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CCK-8 Assay

HZ Huizhen Zhang
SJ Shuangling Jin
AJ Aifang Ji
YM Ying Ma
CZ Chunyan Zhang
AW Ailan Wang
RW Rui Wang
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Cell Counting Kit 8 (ab228554, Abcam) was used to evaluate the effects of transfections on the proliferation of SiHa cells. Each well of a 96-well cell culture plate was filled with 0.1 mL cell suspension containing 5000 cells. Cell culture was performed at 37°C. Measurement of OD values was performed at 450 nm every 24 h for a total of 96 h. CCK-8 solution was added into each well to 10% at 4 h before measuring the OD values.

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