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Intraperitoneal Glucose Tolerance Test (IPGTT) and Insulin Tolerance Test (ITT)

YC Yixin Chen
YC Yu Chen
NW Na Wang
SG Shanhong Gu
MW Meilin Wang
YF Yucai Fu
CW Chiju Wei
WX Wencan Xu
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Blood glucose levels in the tail vein were measured using a Sannuo glucometer (Sannuo, China). We carried out the IPGTT and IPITT at the 20th and 22nd week, respectively. For the IPGTT, mice were fasted for 8 h and the blood glucose was measured at 0, 15, 30, 60, and 120 min after intraperitoneal injection of glucose (1 g/kg bw). For the ITT, mice were fasted for 6 h, then insulin (1 U/kg bw, from Gansulin R, Tonghua Dongbao Pharmaceutical, China) was injected intraperitoneally, and blood glucose was measured at 0, 15, 30, 60, and 120 min.

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