Metascape (www.genemania.org) is a free and well-maintained online bioinformatics database for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis (36). In this study, we used Metascape for functional annotation and pathway enrichment analysis of E2Fs and neighboring genes significantly associated with E2Fs. Only terms with P-value <0.01, minimum count >3, and enrichment factor >1.5 were considered as significant.
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