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NAD+ levels were measured with a NAD/NADH Quantitation Kit (Sigma-Aldrich; Cat# MAK037) according to the manufacturer’s instruction. In brief, 120 oocytes were harvested for total NAD+ extraction and quantification based on the procedure described by Pantazi et al. (2015). The NAD+ concentration was calculated by subtracting the NADH values from NADtotal (NAD+ and NADH). NADtotal and NADH levels were quantified in a colorimetric assay at 450 nm using iMarkTM Microplate Absorbance Reader (Bio-Rad, Hercules, CA, United States).
Copyright and License information: ©2021 Miao, Li, Shi, Gao, Chen, Wang, Fan and Xiong.
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