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One Step growth curve

NS Niranjana Sri Sundaramoorthy
ST Subramaniam Thothathri
MB Muthumeenakshi Bhaskaran
AG ArunKumar GaneshPrasad
SN Saisubramanian Nagarajan
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A one-step growth curve of the phages was performed as reported earlier (Pajunen et al. 2000). The host bacteria was grown until early log phase (0.4 OD595), centrifuged and resuspended in SM buffer. This was mixed with phages at a multiplicity of infection (MOI) of 1 and was allowed to adsorb for 5–10 min at 37 °C. The mixture was then centrifuged, pellet was resuspended in nutrient media and incubated at 37 °C. Samples were withdrawn for every 5 min and were plated to determine phage titer. The experiment was performed in triplicates, and values depict the mean of three observations ± standard deviation. Burst size was calculated as the ratio of average PFU/ml in latent period to the average PFU/ml of last three time points. The experiment was done in triplicates and the error bar represents standard error of the mean.

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