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The siRNAs targeting PARP-7 were from Dharmacon (see below) and the control siRNA (SIC001) was from Sigma. The siRNA oligos were transfected into OVCAR4 cells at a final concentration of 30 nM using Lipofectamine RNAiMAX reagent (Invitrogen, 13778150) according to the manufacturer’s instructions. The cells were used for various assays 24 hr after siRNA transfection. The siRNA oligos used to knockdown PARP7 mRNA were as follows:
PARP7 siRNA 1: D-013948–08 (our siRNA #1)
PARP7 siRNA 2: D-013948–09 (our siRNA #2)
PARP7 siRNA 4: D-013948–07 (our siRNA #3)
Copyright and License information: ©2021, Palavalli Parsons et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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