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Cells were harvested by trypsinization, plated at 50,000 per well in 96 well plates, and allowed to adhere overnight. The next day, cells were washed with PBS and incubated with 50 nM LysoTracker Deep Red and increasing concentrations of propranolol in serum free medium, on ice for 30 min. The cells were washed, and the relative levels of LysoTracker Deep Red were detected using a TECAN Infinite m200 PRO plate reader (ex/em 647/668) (Tecan US, Morrisville, North Carolina).
Copyright and License information: ©2021 Saha, Kim, Amaya, Witcher, Khammanivong, Korpela, Brown, Taylor, Bryan and Dickerson
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