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Screening of mutants with decreased or increased hemolytic activity

BS Bingqing Sun
YX Yafei Xue
XD Xiaoli Du
XH Xiaohua He
ZZ Zuocheng Zou
XT Xiangqiang Tian
ZH Zhonghao Hu
HL Haoyang Liu
NI Nazrul Islam
QH Qinghai Hu
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The hemolytic activities of the wild-type (WT) strain SX and transposon insertion mutants were determined on duck blood agar plates (LB agar base supplemented with 3.5% duck blood, pH 7.5) as described previously [4]. Briefly, suspended bacterial cells were plated on blood agar plates, which were then incubated at 37 °C under an atmosphere of 5% CO2/95% air for 24 h, followed by incubation overnight at 4 °C. The hemolytic zones on duck blood agar generated by the mutants and WT SX strain were compared. Transposon insertion mutants with increased or reduced hemolytic activities were isolated. The hemolytic activities of the mutants were determined at least twice on duck blood agar.

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