Antibodies and Reagents

CAT (purity: 98.3%, HPLC) was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (L20N8Y48597), bleomycin (BLM) from Cool Chemical Technology Co., Ltd. Beijing (S656455V), and PFD from Beijing Continent Pharmaceutical Co., Ltd. (190806). The Masson tricolor staining kit (G1006-100), hematoxylin-eosin (HE) staining kit (GP1031), reactive oxygen species (ROS) test kit (2019-07), detection kits for hydroxyproline (HYP) (201900711), aspartate aminotransferase (AST) (20190712), alanine aminotransferase (ALT) (20190711), malondialdehyde (MDA) (20190830), superoxide dismutase (SOD) (20191125), and the bicinchoninic acid (BCA) method total protein quantification kit (20190711) were all purchased from Nanjing Jiancheng Bioengineering Research Institute. Rabbit anti-Wnt3a antibody (BS-1700r), rabbit anti-phosphorylated-Smad3 antibody (bs-19452r), and rabbit anti-Smad3 antibody (BS-3484r) were purchased from Biosynthesis Biotechnology Inc. Beijing, China. Rabbit anti-α-SMA antibody (GB11044), rabbit anti-matrix metalloprotease (MMP)-7 antibody (A0695), rabbit anti-COL1A1 antibody (GB11022-3), rabbit anti-COL3A1 antibody (GB13023-2), rabbit anti-β-catenin antibody (GB12015), rabbit anti-GSK-3β antibody (GB11099), rabbit anti-E-cadherin (E-cad) antibody (GB13083), and HRP-labeled goat anti-rabbit IgG antibodies (GB23303) were purchased from Wuhan Servicebio Technology Co., Ltd. The rat TGF-β1 enzyme-linked immunosorbent assay (ELISA) kit (E04019240) was purchased from Cusabio Biotech Co., Ltd. Wuhan. Rabbit anti-anti-β-actin antibody (AC026), rabbit anti-phosphorylated-GSK3β antibody (AP0039), and rabbit anti-phosphorylated-β-catenin antibody (AP0979) were obtained from ABclonal Biotechnology Co., Ltd. Wuhan. Rat IL-6 (CSB-E04640r), IL-1β (CSB-E08055r), and TNF-α (CSB-E11987r) ELISA kits were purchased from Wuhan Huamei Bioengineering Co., Ltd.